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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
13/12/2017 |
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Data da última atualização: |
13/12/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BOSETTO, L.; BRANCHER, T. L.; CARLESSO, C.; KVITSCHAL, M. V.; HAWERROTH, M. C. |
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Título: |
The best tissue to obtain DNA from apple tree using CTAB extraction protocol. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON SCIENCE AND BIOTECHNOLOGY, 2., 2017, VIDEIRA. Resumos... Videira: Unoesc, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The DNA extraction is the key for all molecular biology procedures. In plants, this stage is absolutely affected by the tissue compounds, that are variable between the species. In apple tree, polyphenols are the main compounds that affects DNA quality and integrity. The aim of this work was to apply a protocol for DNA extraction using different apple tree tissues and to determine the best one to obtain high DNA quality. The protocol tested was an adaptation of CTAB, using leaf, bud, stem, apple skin and flesh. The experiment was carried out in a completely randomized design with five replicates, considering each tissue. To determinate the best quality of DNA was used the relation 260 nm/ 280nm and 260nm/230nm. Quality parameters and DNA concentration were obtained with absorbance spectrophotometer. It was performed variance analysis and Tukey's test, with a significance level at 5 %. The DNA integrity was observed in agarose gel 0.8 %. The highest mean concentration were 374.53 ng/µL in stem and 395.58 ng/µL in bud tissue. The best quality (260/280 ≈ 2.0, 260/230 ≈ 1.8) was obtain with leaf, bud and stem, the same for DNA integrity. The best tissues to obtain DNA from apple tree with high concentration and quality were bud, stem and leaf, suggesting higher efficiency using this protocol. |
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Palavras-Chave: |
Biotechnology; Deoxyribonucleic acid; Malus sp; Molecular biology. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02009naa a2200217 a 4500
001 1126983
005 2017-12-13
008 2017 bl uuuu u00u1 u #d
100 1 $aBOSETTO, L.
245 $aThe best tissue to obtain DNA from apple tree using CTAB extraction protocol.$h[electronic resource]
260 $c2017
520 $aThe DNA extraction is the key for all molecular biology procedures. In plants, this stage is absolutely affected by the tissue compounds, that are variable between the species. In apple tree, polyphenols are the main compounds that affects DNA quality and integrity. The aim of this work was to apply a protocol for DNA extraction using different apple tree tissues and to determine the best one to obtain high DNA quality. The protocol tested was an adaptation of CTAB, using leaf, bud, stem, apple skin and flesh. The experiment was carried out in a completely randomized design with five replicates, considering each tissue. To determinate the best quality of DNA was used the relation 260 nm/ 280nm and 260nm/230nm. Quality parameters and DNA concentration were obtained with absorbance spectrophotometer. It was performed variance analysis and Tukey's test, with a significance level at 5 %. The DNA integrity was observed in agarose gel 0.8 %. The highest mean concentration were 374.53 ng/µL in stem and 395.58 ng/µL in bud tissue. The best quality (260/280 ≈ 2.0, 260/230 ≈ 1.8) was obtain with leaf, bud and stem, the same for DNA integrity. The best tissues to obtain DNA from apple tree with high concentration and quality were bud, stem and leaf, suggesting higher efficiency using this protocol.
653 $aBiotechnology
653 $aDeoxyribonucleic acid
653 $aMalus sp
653 $aMolecular biology
700 1 $aBRANCHER, T. L.
700 1 $aCARLESSO, C.
700 1 $aKVITSCHAL, M. V.
700 1 $aHAWERROTH, M. C.
773 $tIn: INTERNATIONAL SYMPOSIUM ON SCIENCE AND BIOTECHNOLOGY, 2., 2017, VIDEIRA. Resumos... Videira: Unoesc, 2017.
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Epagri-Sede (Epagri-Sede) |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
28/03/2012 |
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Data da última atualização: |
29/03/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - -- |
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Autoria: |
BALDISSERA, I. T.; ZAMPIERI, S. L.; BAMPI, D. |
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Afiliação: |
Epagri |
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Título: |
Monitoramento da qualidade da água na microbacia tarumanzinho em Águas Frias, SC, BR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
REVISTA DE CIÊNCIAS AMBIENTAIS, Canoas, RS , v. 5, n. 2, p. 5-14, 2011. |
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Idioma: |
Português |
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Notas: |
ISSN, 1981-8858 |
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Palavras-Chave: |
Agua; Aguas Frias; Microbacia Tarumanzinho; Monitoramento; Santa Catarina. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00609naa a2200205 a 4500
001 1006218
005 2012-03-29
008 2011 bl uuuu u00u1 u #d
100 1 $aBALDISSERA, I. T.
245 $aMonitoramento da qualidade da água na microbacia tarumanzinho em Águas Frias, SC, BR.
260 $c2011
500 $aISSN, 1981-8858
653 $aAgua
653 $aAguas Frias
653 $aMicrobacia Tarumanzinho
653 $aMonitoramento
653 $aSanta Catarina
700 1 $aZAMPIERI, S. L.
700 1 $aBAMPI, D.
773 $tREVISTA DE CIÊNCIAS AMBIENTAIS, Canoas, RS$gv. 5, n. 2, p. 5-14, 2011.
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